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  5. Pan Monocyte Isolation Kit, human

Pan Monocyte Isolation Kit, human

MB130-096-537

The Pan Monocyte Isolation Kit is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. This Kit allows the simultaneous enrichment of classical (CD14++ CD16– ), non classical (CD14+ CD16++ ) and intermediate (CD14++ CD16+ ) monocytes. Highly pure unlabeled monocytes are obtained by depletion of the magnetically labeled cells. For research use only. Size: for 1×10^9 total cells

Brand: Miltenyi Biotec

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Detailed separation procedure
Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads.
Downstream applications

  • Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
  • Studies on monocyte activation, differentiation, cytokine secretion etc.
  • Studies on signal transduction in monocytes.
  • Studies on antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
  • In vitro differentiation of blood monocytes into dendritic cells.

Columns
MS, LS, XS, or autoMACS® Columns.