Cell biology 

The kit contains NucView™ 488 Caspase-3 substrate and a caspase-3 inhibitor Ac-DEVD-CHO sufficient for 100 flow cytometry or microplate assays (200 uL assay volume). The trial size kit contains sufficient reagents for 25 assays (200 uL assay volume). The number of fluorescence microscopy assays that can be performed with the kit may vary based on the size of culture vessel and staining volume used. The kit provides a convenient tool for profiling apoptotic cell population based on caspase-3 activity using either fluorescence microscopy or flow cytometry.

Kit Components:

• NucView™ 488 Caspase-3 substrate, 0.2 mM in DMSO
• Caspase-3 inhibitor Ac-DEVD-CHO, 2 mM in DMSO

Features:

• Real time caspase-3 detection in intact cells: Detect caspase-3 activity within individual cells in a population.
• Bi-functional: Allows visualization of caspase-3 activity and apoptotic nuclear morphology at the same time.
• Simple & Fast: 15-minute assay with no wash required.
• Versatile: For detection by flow cytometry, fluorescence microscopy, or fluorescence microplate reader using fluorescein detection settings.
• Fixable: Compatible with standard fixation and permeabilization methods for subsequent immunofluorescence staining.