Cell biology 

Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and accessories for washing away or quenching extracellular dye fluorescence. Kits are available with SynaptoGreen™ C4 (equivalent to FM1-43), the fixable green nerve terminal dye AM1-43, or SynaptoRed™ C2 (equivalent to FM4-64). ADVASEP-7 is a sulfonated β-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (FM1-43) to stain brain slices. SCAS (4-Sulfonato calix[8]arene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps. Sulforhodamine 101 has been used as a quencher dye to reduce SynaptoGreen™ C4 fluorescence.

Please see the attached nerve terminal probes flyer for detailed information.