Cell biology 

MitoView™ dyes are available in three colors: MitoView™ Blue, MitoView™ Green, and MitoView™ 633.

MitoView™ Blue is a blue fluorescent mitochondrial dye with absorbance/emission at 398/440 nm, suitable for detection by confocal microscopy or flow cytometry using settings for DAPI or Pacific Blue®. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. The dye is designed for use in live cells and is not fixable. Note: MitoView™ Blue may not be membrane potential-dependent in yeast cells.

MitoView™ Green is a green fluorescent mitochondrial dye with properties similar to those of MitoTracker® Green FM. The dye is nonfluorescent until it partitions into the mitochondrial membrane. The staining relies on mitochondrial mass, not on mitochondria membrane potential. Thus, the dye can be used to stain mitochondria in both live cells and fixed cells. MitoView™ Green is spectrally similar to FITC, making it optimally excitable by the 488 nm argon laser line. Note: MitoView™ Green appears to be somewhat membrane potential-dependent in yeast cells.

MitoView™ 633 is a far-red fluorescent mitochondrial dye with absorbance/emission at 622/648 nm. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. The dye is designed for use in live cells, and is not fixable. Note: MitoView™ 633 may not be membrane potential-dependent in yeast cells.

Available in Trial size (50 ug) or 20 x 50 ug.