LysoView 633 is a far-red stain for imaging lysosome localization and morphology in live cells. Lysosomotropic dyes like LysoView™ 633 possess weakly basic amine groups. It is hypothesized that protonation of weak bases under acidic conditions causes the dyes to be trapped in the low pH compartment of the lysosome. In addition, the fluorescence of LysoView™ 633 is pH-sensitive, resulting in highly specific lysosomal staining without the need for a wash step.
LysoView dyes label lysosomes in live cells, and are available in 3 dye colors: visible red LysoView™ 540, far-red LysoView™ 633, and far-red LysoView™ 650.
Packaging size:
10 vials of lyophilized dye. One vial yields 100 uL of 1000X dye stock solution after reconstitution.