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  5. IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

MB130-090-435

The IL-10 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-10-secreting cells. For research use only. Size: for 50 tests

Brand: Miltenyi Biotec

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MB130-090-435 IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human Login for price  

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Background information
Interleukin-10 (IL-10) is predominantly secreted by human CD4+ T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by TH 1 cells, activated monocytes, and NK cells. It can stimulate the immunoglobulin production by B cells.
Downstream applications
IL-10 Secretion Assays were used to analyze IL-10-secreting, CMV-specific CD4+ T cells after stimulation with CMV lysate. The IL-10 Secretion Assay was also used to analyze IL-10 secretion by allergen-specific T cells. Furthermore, it was used for the detection and isolation of antigen-specific IL-10-secreting type I regulatory T cells from patients with an autoimmune skin disease. The isolated cells were cloned, expanded and further analyzed for their regulatory function.
Columns
MS, LS, or autoMACS® Columns.