Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.
- Evagreen® dye: Evagreen® DNA-binding dye selectively binds to dsDNA via a novel “release-on-demand” mechanism that ensures low PCR inhibition and thus permits the dye to be used at a relatively high concentration for melt curve analysis signal. Because the dye is spectrally similar to FAM or SYBR® Green I, the dye is compatible with all commercial qPCR instruments. Moreover, unlike SYBR® Green I and SYBR GreenER®, Evagreen® dye is extremely stable. Finally, Evagreen® dye is nonmutagenic and noncytotoxic.
- Cheetah™ Taq: Cheetah™ Taq is a new, chemically modified hotstart DNA polymerase. Unlike Amplitaq Gold®, Cheetah™ Taq can be activated in as little as two minutes under standard hotstart condition (i.e., 94°C in pH 8-9 Tris) with high activity recovery. Also unlike antibody-based hotstart polymerases, such as Platinum® Taq, Cheetah™ Taq is completely inactive at room temperature and has intrinsically low DNA contamination. In addition, Cheetah™ Taq is generally more stable at -20°C or 4°C than AmpliTaq Gold®, ensuring that the master mix maintains its peak performance following storage at low temperature.
Fast Evagreen® qPCR Master Mix is a 2X master mix, and is supplied with a separate vial of ROX reference dye, for use with high ROX, low ROX, or no ROX instruments.
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