Dyes and Fluorophores 

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3′- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF™ dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry. 

CF488A dye offer exceptional brightness and photostability.

• Ex/Em: 490/515 nm

Kit Components:

• TUNEL Equilibration Buffer: 5 mL
• TUNEL Reaction Buffer: 5 x 0.5 mL
• TdT Enzyme: 50 uL