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  5. Classical Monocyte Isolation Kit, human

Classical Monocyte Isolation Kit, human

MB130-117-337

The Classical Monocyte Isolation Kit provides fast and consistent isolation of untouched classical ( CD14++ CD16– ) monocytes from peripheral blood mononuclear cells (PBMCs) for further functional studies. Depletion of non-monocytes is optimal and provides a highly pure population of label-free classical monocytes. This product includes an optional thrombocytes removal reagent to further reduce platelet contamination. For research use only. Size: for 1×10^9 total cells

Brand: Miltenyi Biotec

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Background information
Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled monocytes is achieved by depletion of the magnetically labeled cells.

Downstream applications

  • Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
  • Studies on monocyte activation, differentiation, cytokine secretion etc.
  • Studies on compound or antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
  • In vitro
    • differentiation of blood monocytes into dendritic cells or macrophages.

Columns
MS Columns, LS Columns, autoMACS® Columns, or Multi-24 Column Blocks.