Cell Separation 

Background information
CD127, the α-chain of the IL-7 receptor, is a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc). CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation. On regulatory T cells CD127 is absent and its expression is inversely correlated with FoxP3 expression and suppressive function. CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.
Human regulatory T cells can be isolated, for example, by using the CD4+ CD25+ CD127dim/– Regulatory T Cell Isolation Kit, human.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.