Background information
The FITC molecule is recognized by the monoclonal anti-FITC antibody coupled to MicroBeads, allowing the positive selection or depletion of the fluorescently labeled material with MACS® Technology. Labeling samples with FITC-conjugated primary antibodies and Anti-FITC MicroBeads enables subsequent flow cytometric analysis of separated cells without any further staining of the material.
Applications
Examples among the many separations performed with Anti-FITC MicroBeads are the isolation of IgD+ B cells from human PBMCs, the separation of plant protoplasts as well as the isolation of murine Langerhans cells from epidermal tissue. Anti-FITC MicroBeads have also been used for the depletion of CD3+ cells from rat spleen and subsequent isolation of CD3- NK cells or the separation of murine naive CD8+ T cells expressing low levels of CD44 by depletion of CD44int/high cells.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.