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  6. Treg Expansion Kit, human

Treg Expansion Kit, human

The Treg Expansion Kit was developed for the efficient expansion of human Treg cells. For research use only

Brand: Miltenyi Biotec

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Product CodeProduct Product Description Price (Excl gst) Qty Add
MB130-095-345 Treg Expansion Kit, human, 2 mL Treg Expansion Kit, human, 2 mL Login for price  
MB130-095-353 Treg Expansion Kit, human, 2×2 mL Treg Expansion Kit, human, 2×2 mL Login for price  

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Background information
Using this kit FoxP3 expression by Treg cells is sustained after isolation with the CD4+ CD25+ Regulatory T cell Isolation Kit, CD4+ CD25+ CD127dim/– or CD4+ CD25+ CD45RA+ Regulatory T Cell Isolation Kits from human blood or PBMCs. The kit is also suitable for usage with rapamycin.
The Treg Expansion Kit is based on MACSiBead™ Particles pre‑loaded with CD3 and CD28 antibodies. Optimal expansion of Treg cells is accomplished by using pre-loaded MACSiBead Particles and Treg cells at a bead-to-cell ratio of 4:1 and recombinant interleukin 2 (rIL-2) with a concentration of 500 U/mL.

Downstream applications
Expansion of human Treg cells after isolation with the CD4+ CD25+ CD127dim/– or CD4+ CD25+ CD45RA+ Regulatory T Cell Isolation Kits from human blood or PBMCs. Expanded Treg cells can be used for any downstream application such as cytokine analysis, gene expression, and suppression assays.