Background information
StemMACS™ CHIR99021 is a highly selective inhibitor of glycogen synthase kinase 3 (GSK-3), a crucial regulator of the Wnt signaling pathway. The aminopyridine CHIR99021 inhibits both GSK-3 isoforms, GSK-3α (IC50 10 nM) and GSK-3β (IC50 6.7 nM). Unlike other GSK-3 inhibitors, it does not cross-react with cyclin-dependent kinases (CDKs). Activation of Wnt signaling via CHIR99021-mediated GSK-3 inhibition is widely used to modulate pluripotent stem cell differentiation and self-renewal.
Applications
Published applications for CHIR99021 include:
- Naïve, ground state culture of murine ES and iPS cells using 2i conditions
- Naïve, ground state culture of rat ES and iPS cells using 3i conditions
- Naïve, ground state culture of human ES and iPS cells
- Differentiation of human ES and iPS cells into cardiomyocytes
- Differentiation of human ES and iPS cells into skeletal muscle
- Differentiation of human ES and iPS cells into definitive endoderm
- Chemical Reprogramming of mouse fibroblasts
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