Cell Culture 

Applications
Human IFN-γ1b can be used for a variety of applications, including:

• Stimulation of monocytes, macrophages, and microglia.
• In vitro maturation of Mo-DCs.
• Apoptosis and cell growth inhibition assays.
• Adhesion and migration assays.

Alternative names
IFN-γ

Background information
IFN-γ, the only type II interferon, possesses potent anti-viral activity and immunoregulatory properties. It is structurally unrelated to the type I interferons and binds to a different receptor expressed by most immune cells. IFN-γ is produced predominantly by NK cells and T cells. IFN-γ enhances NK cell and macrophage activity and regulates B cell functions, like immunglobulin production and class switching. In addition, IFN-γ orchestrates leukocyte attraction and directs growth, maturation, and differentiation of many cell types. The activity of human IFN-γ is species-specific.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity
Proliferation of HT-29 cells (NIAID Gxg01-902-535)
premium grade: ≥ 2×10⁷ IU/mg (typical activity: ≥ 5×10⁷ IU/mg)
research grade: ≥ 1×10⁷ IU/mg