Clone AD5-14H12 recognizes the human CD141 (BDCA-3) antigen which is expressed at high levels on a minor subpopulation of human myeloid dendritic cells (about 0.02% of blood leukocytes). CD141 (BDCA‑3)high blood dendritic cells are CD11cdim , CD123– , CD4+ , Lin– , CD45RO+ , CD2– , and CD16– . They express myeloid lineage markers, such as CD13 and CD33, and have a monocytoid morphology. Unlike CD1c (BDCA-1)+ blood dendritic cells, CD141 (BDCA-3)high blood dendritic cells lack expression of CD2 and Fc receptors such as CD32, CD64, or FcεRI. CD141 (BDCA-3) is also present at very low levels on CD14+ monocytes, granulocytes, CD303 (BDCA‑2)+ CD304 (BDCA-4/Neuropilin-1)+ plasmacytoid and CD1c (BDCA-1)+ myeloid dendritic cells. CD141 (BDCA‑3)high CD1c (BDCA-1)– myeloid dendritic cells have been designated type-2 myeloid dendritic cells (MDC2s). CD141 is also known as thrombomodulin; thrombomodulin mediates co-agglutination by interaction with thrombin and protein C, though nothing is known about its function on MDC2s.
Alternative names
THBD, AHUS6, BDCA3, THPH12, THRM, TM
Technical specifications
Antigen: CD141 (BDCA-3)
Clone: AD5-14H12
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Alternative names of antigen: THBD, AHUS6, BDCA3, THPH12, THRM, TM
Entrez Gene ID: 7056
Molecular mass of antigen [kDa]: 59
Distribution of antigen: endothelial cells, megakaryocytes, monocytes, myeloid cells, neutrophils, platelets, smooth muscle
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: APC-Vio770, Biotin, pure, FITC, VioBright FITC, PE, APC, VioBlue, PE-Vio770
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