Cell biology 

Background information
The CD43 antigen is expressed on nearly all leukocytes except immature and mature resting B cells.
Detailed separation procedure
By using CD43 MicroBeads, all CD43-expressing cells are magnetically labeled, whereas the resting B cell subset stays untouched and is collected in the column effluent.
Downstream applications
Resting B cells isolated with CD43 MicroBeads have been used for various studies on B cell differentiation and related molecular processes. Resting B cells isolated from spleen were used for in vitro analyses on antibody class switching. They were separated for comparative analysis on B cell receptor signal transduction in wild type and immunodeficient mice or were used as starting material for studies on germinal center formation.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.