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  5. CD3-Biotin - functional grade, human

CD3-Biotin - functional grade, human

MB130-093-377

The CD3 antibody recognizes the human CD3 antigen which is present on mature human T cells, thymocytes, and NKT cells. The epitope recognized by the antibody is located on the ε-chain of the CD3 complex. Clone OKT3 has been shown to activate T cell function. For research use only. Size: 100 µg in 1 mL

Brand: Miltenyi Biotec

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Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of antigen-presenting cells (APCs). The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency.
Applications
The CD3 antibodies – functional grade are suited for functional assays, for example, in vitro activation and expansion of T cells in combination with CD2 and CD28 pure – functional grade antibodies, or CD2- and CD28-Biotin – functional grade antibodies, and Anti-Biotin MACSiBead™ Particles, cell culture grade.
Alternative names
CD3e, IMD18, T3E, TCRE

Antigen: CD3
Clone: OKT3
Isotype: mouse IgG2aκ
Alternative names of antigen: CD3e, IMD18, T3E, TCRE
Entrez Gene ID: 915, 916, 917
Molecular mass of antigen [kDa]: 56
Distribution of antigen: NK cells, T cells, thymocytes
Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: pure