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  5. CD28-Biotin - functional grade, human

CD28-Biotin - functional grade, human

MB130-093-386

The CD28 antibody recognizes the human CD28 antigen, which is highly expressed on CD3+ thymocytes, most peripheral thymocytes, and plasma cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production in vitro and in vivo. In the presence of antibodies directed against CD2 and CD3, CD28 antibodies stimulate T cell proliferation and cytokine production.For research use only. Size: 100 µg in 1 mL

Brand: Miltenyi Biotec

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Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of antigen-presenting cells (APCs). The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency.
Applications
The CD28 antibodies – functional grade are suited for functional assays, for example, in vitro activation and expansion of T cells in combination with CD2 and CD3 pure – functional grade antibodies, or CD2- and CD3-Biotin – functional grade antibodies, and Anti-Biotin MACSiBead™ Particles, cell culture grade.
Alternative names
Tp44, T44

Antigen: CD28
Clone: 15E8
Isotype: mouse IgG1
Alternative names of antigen: Tp44, T44
Entrez Gene ID: 940
Molecular mass of antigen [kDa]: 23
Cross-reactivity: rhesus monkey ( Macaca mulatta ), cynomolgus monkey ( Macaca fascicularis )
Distribution of antigen: B cells, lymphocytes, plasma cells, T cells, thymocytes
Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: pure