MojoSort 

Kit Contents

For BIO480059:

• 200 µl of of Biotin-Antibody Cocktail: Biotin anti- CD3, CD7, CD15, CD19, CD20, CD56, CD57, CD123, CD235ab
• 200 µl Streptavidin Nanobeads
• 1 vial containing 100 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution)

For BIO480060:

• 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD3, CD7, CD15, CD19, CD20, CD56, CD57, CD123, CD235ab
• 2 vials of 1 ml Streptavidin Nanobeads each
• 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)

Reactivity Human

Formulation Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.

Preparation The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Strepavidin Nanobeads: Streptavidin-coated magnetic beads.

Storage & Handling

Antibody cocktail, Streptavidin Nanobeads, and Human TruStain FcX™ (Fc Receptor Blocking Solution) should be stored undiluted between 2°C and 8°C.

Application Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.

10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.

5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution) for 1 x 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched pan monocytes from peripheral blood mononuclear cells (PBMCs).