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Living Colors® A.v. Monoclonal Antibody (JL-8)

A monoclonal antibody produced by hybridoma cells against full-length Aequorea victoria green fluorescent protein (GFP). This antibody recognizes native and denatured forms of wild-type GFP, GFPuv, AcGFP, EGFP, destabilized EGFP variants, EBFP, EYFP, ECFP, AcGFP, and both N- and C-terminal fusion proteins containing these GFP variants in bacterial and mammalian cell lysates.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT632381 Living Colors® A.v. Monoclonal Antibody (JL-8) 200 µl Living Colors® A.v. Monoclonal Antibody (JL-8) 200 µl Login for price  
CLT632380 Living Colors® A.v. Monoclonal Antibody (JL-8) 20 µl Living Colors® A.v. Monoclonal Antibody (JL-8) 20 µl Login for price  

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Recommend Applications & Suggested Dilutions

  • This antibody is recommended for Western blotting and immunoprecipitation.
  • For Western blotting, we suggest using the antibody at a 1:1,000 to 1:8,000 dilution.
  • For immunoprecipitation, we suggest you titrate the amount of antibody needed for quantitative recovery of the antigen. As a starting point, however, we recommend using the antibody at a 1:50 to 1:100 dilution. Analyze the immunoprecipitate by Western blotting with our Living Colors Full-Length GFP Polyclonal Antibody (Cat. Nos. 632592 & 632593).

Package Contents

  • 1 tube Living Colors A.v. Monoclonal Antibody (JL-8)

Form

  • Affinity-purified monoclonal antibody (mouse IgG2a isotype) in Phosphate Buffered Saline, pH 7.2, filter-sterilized

Concentration

  • 1 mg/ml

Storage Conditions

  • Store at –20°C.
  • Avoid repeated freeze/thaw cycles.

Shelf Life

  • 1 year from date of receipt under proper storage conditions.