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APC/Fireª 750 anti-human CD20

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Brand: Biolegend

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Product CodeProduct Product Description Price (Excl gst) Qty Add
BIO302358 APC/Fireª 750 anti-human CD20 100 tests APC/Fireª 750 anti-human CD20 100 tests Login for price  
BIO302357 APC/Fireª 750 anti-human CD20 25 tests APC/Fireª 750 anti-human CD20 25 tests Login for price  

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Isotype Control APC/Fire™ 750 Mouse IgG2b, κ Isotype Ctrl

Reactivity Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey

Immunogen Human tonsillar B cells Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.

Concentration Lot-specific (please contact technical support for concentration and total µg amount)

Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application FC - Quality tested

Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Size 25 or 100 tests