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  5. Alexa Fluor® 647 anti-H2A.X Phospho (Ser139),100 tests - BIO613408

Alexa Fluor® 647 anti-H2A.X Phospho (Ser139),100 tests - BIO613408

BIO613408

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for DNA repair and maintaining genomic stability and for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry.

Brand: Biolegend

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Isotype Control Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC)

Reactivity Human, Mouse

Immunogen Modified peptide

Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.

Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)

Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application ICFC - Quality tested
IF - Reported in the literature

Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

Excitation Laser Red Laser (633 nm)

Size 100 tests