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  7. 7-AAD Viability Staining Solution, 500 tests - BIO420404

7-AAD Viability Staining Solution, 500 tests - BIO420404

BIO420404

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Brand: Biolegend

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Formulation PBS, pH 7.2 with 0.09% (w/v) sodium azide.

Preparation CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.

Concentration 50 µg/ml

Storage & Handling Protect from light. Store between 2°C and 8°C.

Application FC - Quality tested

Recommended Usage For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.
We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

Excitation LaserBlue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Size 500 tests