Molecular and Microbiology Reagents 

5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation.

• λ_Ex/λ_Em (of hydrolysis product at pH 7) = 495/519 nm
• White solid soluble in DMSO or DMF
• Store at -20°C and protect from light
• C₂₉H₁₉NO₁₁
• MW: 557
• Size: 25mg