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  5. vinoBRETT kit

vinoBRETT kit

Managing Brettanomyces proactively through onsite analysis at the winery. vinoBRETT is game-changing detection technology than brings affordable, onsite PCR testing to your winery. vinoBRETT makes it possible to conduct routine, proactive testing throughout the winemaking process to obtain accurate information about the presence and level of Brettanomyces at any time.

Brand: Invisible Sentinel

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Product CodeProduct Product Description Price (Excl gst) Qty Add
OT-IS0508200 VERIFLOW BRETT PCR REAGENT KIT 24T VERIFLOW BRETT PCR REAGENT KIT 24T Login for price  
OT-IS6014 VERIFLOW VINOBRETT KIT 24T VERIFLOW VINOBRETT KIT 24T Login for price  
OT-ISEV012 BRETT POSITIVE CONTROL 200ul /40T BRETT POSITIVE CONTROL 200ul /40T Login for price  

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Invisible Sentinel’s wine quality products are powered by Veriflow® - a game-changing platform technology that combines proven diagnostic principles for microbial detection and innovative, first-in-class scientific approaches. Veriflow technology offers unparalleled performance with no compromise in ease-of-use or affordability.

The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative test results are obtained in less than 4 hours via hand-held cassette, and quantitative results can be tracked, stored, and exported via the Veriflow Reader.

Early detection at low levels

Using vinoBRETT, winemakers identify the presence of Brettanomyces long before the effects of 4-EP/EG exceed the sensory detection threshold. Brettanomyces management begins as early as the fermentation stage, which allows for proactive management with less intrusive methods before extensive or damaging remediation measures are required.

  • Robust detection even in young wines
  • Identify at-risk barrels to isolate and prevent cross-contamination

Speed, accuracy and sensitivity

Veriflow technology is AOAC International Certified for foodborne pathogen detection. vinoBRETT was developed specifically for wine makers and has been validated and implemented by a variety of award-winning, quality-centric wineries around the world.

  • Internal and external laboratory validation in collaboration with Jackson Family Wines and Enartis Vinquiry
  • 100% inclusive of 49 known isolates of Brettanomyces bruxellensis1
  • Correctly excluded 100% of multiple species of commonly occurring microorganisms found in wine

1 Strains sourced for University of California-Davis Wine Microbe Collection, Department of Viticulture & Enology

Simple and cost-efficient deployment

vinoBRETT is ideally suited for use in the winery – with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by enologists and winemakers with minimal training.

  • The systems quantifies viable Brettanomyces populations in less than 4 hours
  • Requires only minutes of hands-on time to perform the test

Veriflow DNA Signature Capturing Technology

DNA Amplification Proprietary reagents eliminate need for sample purification
DNA Identification Proprietary DNA signature detection specifically targeting Brettanomyces
Visualization of Results Vertical flow mediated visualization of results for easy interpretation via hand-held cassette
Sample Preparation No enrichment or purification steps required
Certification AOAC International Certified for foodborne pathogen detection
Current Utilization Food and juice manufacturers, premium wineries, craft breweries; global 3rd party testing labs; U.S. and international

vinoBRETT Performance Specifications

Sensitivity (LOD) 10 cells/ml
Time to Results < 4 hours
Matrix Compatibility Juice, wine, lees, barrel rinsate, colony PCR, enrichment broth
Assay Configuration Qualitative and quantitative
Target Selection Ribosomal Deoxyribonucleic Acid (rDNA) gene
Specificity Brettanomyces bruxellensis
Active state and VBNC state (Viable But Non-Culturable)

vinoBRETT Validation

Validation Studies Internal and external laboratory validation in collaboration with Jackson Family Wines and Enartis Vinquiry
Inclusivity 100%. Correctly identified 49 known isolates of Brettanomyces bruxellensis1
Exclusivity 100%. Correctly excluded multiple species of commonly occurring microorganisms found in wine

1 Strains sourced from University of California-Davis, Wine Microbe Collection, Department of Viticulture & Enology