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The Normalized Mate & Plate Libraries are high-complexity cDNA libraries cloned into a GAL4 AD vector and transformed into yeast strain Y187. These libraries significantly reduce the labor and time required to perform a yeast two-hybrid screen because library amplification and yeast transformation have already been done. Additionally, normalization selectively removes highly abundant transcripts from the libraries to enhance the representations of low-abundance and rare cDNAs.
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