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  4. Neural Tissue Dissociation Kits

Neural Tissue Dissociation Kits

The Neural Tissue Dissociation Kits have been developed for the gentle and efficient generation of single-cell suspensions from neural tissues. Depending on the application the kits can be used with manual mechanical dissociation or with automated dissociation using the gentleMACS™ Dissociators. For research use only. Size: for 50 preparations

Brand: Miltenyi Biotec

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Product CodeProduct Product Description Price (Excl gst) Qty Add
MB130-092-628 Neural Tissue Dissociation Kit - Papain Neural Tissue Dissociation Kit - Papain Login for price  
MB130-093-231 Neural Tissue Dissociation Kit - Trypsin Neural Tissue Dissociation Kit - Trypsin Login for price  

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Background information
In less than an hour, the gentle two-step enzymatic dissociation procedure efficiently yields high numbers of viable cells. The Neural Tissue Dissociation Kit (P) contains papain, and the Neural Tissue Dissociation Kit (T) contains trypsin.
Some antigen epitopes are damaged by papain, others by trypsin. The kit based on papain is best suited for use with Anti-Prominin-1 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, Anti-GLAST (ACSA-1) MicroBead Kit and Anti-PSA-NCAM MicroBeads require the use of the trypsin-based kit.
Please see figure 1 for further information with regard to epitope sensitivity and kit selection.