Next Gen Sequencing Kits 

The EpiXplore Meth-Seq DNA Enrichment Kit is a methylated DNA enrichment and library prep kit. This is a product for separating the methylated and unmethylated fractions of DNA, and generating sequencing-ready Illumina® libraries from sheared genomic DNA. This kit utilizes his-tagged MBD2 protein and CapturemTM Nanoprep columns to separate methylated DNA from unmethylated DNA in ~2 hours, and employs our DNA SMARTTM technology to generate indexed meth-seq libraries suitable for NGS on Illumina sequencing platforms. Sequencing libraries are generated without the need for enzymatic cleanup or additional adapter ligations. All necessary reagents for producing meth-seq libraries are provided in this kit, including primer sets that contain Illumina index sequences.

Overview

• Rapid, user-friendly protocol which separates methylated DNA from unmethylated DNA
• Ligation-independent sequencing-adapter addition for library preparation
• DNA inputs of 25 ng–1 μg sheared genomic DNA
• Generate Illumina-ready sequencing libraries from the enriched DNA in ~4 hours

Applications

• Sequencing library preparation for methylated DNA-seq from low-input, sheared genomic DNA
• Illumina-specific NGS sequencing library production