Enjoy FREE SHIPPING on all FreeStyle Libre 2 purchases until 31 May 2024. Simply enter promo code FREESHIP at checkout and click ‘SUBMIT CODE’ to apply!

Pharmac proposes to commence FreeStyle Libre 2 subsidy for people living with type 1 diabetes! Read more

Order pick-ups are not available

X
Menu
Cart
X

Search Website

QUICK ORDER PRODUCTS

If you know the product code enter it here  

  1. Home
  2. Laboratory
  3. Molecular and Microbiology Reagents
  4. Molecular
  5. Next Gen Sequencing Kits
  6. ChIP Elute Kit and DNA SMART ChIP-Seq Kit

ChIP Elute Kit and DNA SMART ChIP-Seq Kit

The ChIP Elute Kit uses a fast and simple method to reverse cross-link, elute, and purify DNA captured by chromatin immunoprecipitation (ChIP). This kit replaces long and tedious protocols for the final stages of ChIP experiments. Single-stranded DNA (ssDNA) can be recovered in approximately one hour (compared to traditional methods that may require overnight incubation) and is directly compatible with the DNA SMART ChIP-Seq Kit.

Brand: Takara

Learn more

Select your options below
Enquire about this product
Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT634887 ChIP Elute Kit, 50 Rxns ChIP Elute Kit, 50 Rxns Login for price  
CLT634865 DNA SMART™ ChIP-Seq Kit - 12, 12 Rxns DNA SMART™ ChIP-Seq Kit - 12, 12 Rxns Login for price  
CLT634866 DNA SMART™ ChIP-Seq Kit - 48 A, 48 Rxns DNA SMART™ ChIP-Seq Kit - 48 A, 48 Rxns Login for price  
CLT634867 DNA SMART™ ChIP-Seq Kit - 48 B, 48 Rxns DNA SMART™ ChIP-Seq Kit - 48 B, 48 Rxns Login for price  

Go to checkout

X

Stock Request

Submit your details.
You are enquiring about ChIP Elute Kit and DNA SMART ChIP-Seq Kit

Pricing Request Starts Here

Submit your details to receive a quote.
You are enquiring about ChIP Elute Kit and DNA SMART ChIP-Seq Kit

Overview

  • Fast (one hour) cross-link reversal and elution of ChIP DNA
  • Ligation-independent adapter addition for ChIP-seq library preparation
  • Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA
  • Generate Illumina-ready sequencing libraries in around 4 hours

There are three versions of this kit available:

  • The DNA SMART ChIP-Seq Kit - 12 contains the reagents to generate up to 12 indexed libraries using a single forward indexing primer and 12 reverse indexing primers
  • The DNA SMART ChIP-Seq Kit - 48 A can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers
  • The DNA SMART ChIP-Seq Kit - 48 B can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers

Note: DNA SMART ChIP-Seq Kit - 48 A and DNA SMART ChIP-Seq Kit - 48 B use different sets of forward primers with different index sequences

ChIP sequencing (ChIP-seq) combines chromatin immunoprecipitation (ChIP) with next-generation sequencing (NGS) to identify the location of protein binding to DNA. The DNA SMART ChIP-Seq Kit generates indexed ChIP-seq libraries suitable for NGS on Illumina sequencing platforms. Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Clontech's DNA SMART technology; sequencing libraries are generated without the need for enzymatic cleanup or adapter ligations. All of the necessary reagents for producing ChIP-seq libraries are provided in this kit, including primer sets that contain Illumina high-throughput indexes. There are three different versions of the kit depending on the number and specific Illumina indexes desired.

The ChIP Elute Kit is designed to be used downstream of a chromatin immunoprecipitation (ChIP) assay to elute DNA
associated with the antibody. The ChIP Elute Kit allows for simultaneous elution and crosslinking reversal of nuclear protein-DNA complexes in a single step. The resulting DNA is single stranded (ssDNA) and is purified and concentrated with the ChIP-grade reagents provided in the kit. The output from this kit is directly compatible with ChIP-seq library preparation using the DNA SMART ChIP-Seq Kit. The DNA SMART ChIP-Seq Kit generates sequencing libraries compatible with Illumina platforms from single-stranded or double-stranded fragmented DNA.

Required Products

  • CLT638504 SeqAmp™ DNA Polymerase, 50 Rxns
  • CLT638509 SeqAmp™ DNA Polymerase, 200 Rxns