Expression Vectors 

Cotransfection of a Linear Selection Marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker (1). Linear selection markers are cotransfected at a decreased ratio relative to the expression vector (i.e., 20-fold less of the linear marker).

The pTK-Hyg Selection Vector and the pPur Vector may be used to select for stably transformed mammalian cells using hygromycin and puromycin, respectively. When cotransfected with pTRE2 or a pBI vector, pTK-Hyg is especially useful for selecting double-stable Tet-On or Tet-Off Cell Lines. When integrated into the host cell’s genome, pTK-Hyg does not cause unwanted activation of pTRE- and pBI-derived plasmids because the HSV TK promoter lacks an enhancer element.

Features

• Both the Linear Selection Markers and the pTK-Hyg Selection Vector and pPur Vectors may be used in cotransfections to establish stable cell lines
• Linear Selection Markers require you to screen fewer clones, resulting in more positive clones than when circular plasmid markers are used
• Linear Selection Markers are ideal for use with all Clontech's Tet-On and Tet Off Inducible
• Expression Systems—screen less and find more highly inducible clones