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  5. Enzymes
  6. Cryonase Cold-Active Nuclease

Cryonase Cold-Active Nuclease

Cryonase Cold-Active Nuclease is a recombinant endonuclease originally derived from a psychrophile, Shewanella sp., and purified from E. coli. This endonuclease enzyme can complete digestion of all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) at cold temperatures and even in samples placed on ice. As a result, Cryonase Cold-Active Nuclease is frequently used during DNA digestion of mixed samples, such as those that include heat-labile proteins.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
TK2670A Cryonase™ Cold-Active Nuclease 10,000 Units Cryonase™ Cold-Active Nuclease 10,000 Units Login for price  
TK2670B Cryonase™ Cold-Active Nuclease 50,000 Units Cryonase™ Cold-Active Nuclease 50,000 Units Login for price  

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Applications

  • Digestion of all types of DNA
  • Digestion of all types of RNA
  • Enzymatic and other heat-labile reactions

Purity

  • Protease activity is less than the detection limit
  • E. coli genomic DNA contamination is less than the detection limit

Buffer

Supplied in a buffer (20 mM NaCl, 10 mM Tris-HCl (pH 7.5), 50% glycerol, 2mM MgCl2)

Product citations

Baldermann, S. et al. Functional characterization of a carotenoid cleavage dioxygenase 1 and its relation to the carotenoid accumulation and volatile emission during the floral development of Osmanthus fragrans Lour. J. Exp. Bot. 61(11):2967–2977 (2010).

Yang, H. et al. Full Genome Sequence of Bluetongue Virus Serotype 4 from China. J. Virol. 86(23):13122–13123 (2012).