Cell Separation 

Background information
Neutrophils are essential for host defense against invading pathogens. They are phagocytic polymorphonuclear cells, which engulf and degrade microorganisms using a wide range of toxic agents including reactive oxygen species, antimicrobial peptides, and proteases. Besides, neutrophils can produce anti-inflammatory molecules and factors that promote the resolution of inflammation.
Detailed separation procedure
Non-neutrophils are incubated with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion.
Downstream applications
Functional studies on neutrophils.
Studies on neutrophil activation, differentiation, cytokine secretion etc.
Studies on signal transduction in neutrophils.
Columns
LS Columns for neutrophil isolation from bone marrow, MS Columns for neutrophil isolation from peripheral blood, or autoMACS® Columns.