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  6. Naive CD4+ T Cell Isolation Kit II, human

Naive CD4+ T Cell Isolation Kit II, human

MB130-094-131

The Naive CD4+ T Cell Isolation Kit II was developed for the isolation of untouched naive CD4+ T cells from human peripheral blood. For research use only. Size: for 1×10^9 total cells

Brand: Miltenyi Biotec

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Detailed separation procedure
For magnetic depletion of memory CD4+ T cells and non-CD4+ T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.
Downstream applications
Naive CD4+ T cells isolated with the Naive CD4+ T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs. Untouched naive CD4+ T cells can also be used for further isolation of specific T helper cell subsets.
For isolation of naive CD4+ recent thymic emigrants the CD4+ Recent Thymic Emigrant Isolation Kit, human is recommended.
Columns
LS, XS, or autoMACS® Columns.