Background information
The Mouse Cell Depletion Kit has been designed for the enrichment of untouched human cells upon xenotransplantation. Human tumor xenografts represent the benchmark for research areas like drug discovery, cancer stem cell biology, and metastasis prediction. During the growth phase in vivo , xenografted tissue is vascularized and infiltrated by cells of mouse origin, including heterogeneous lymphocyte subpopulations, fibroblasts, and endothelial cells. The level of infiltration is highly dependent on multiple factors like tumor subtype, growth rate, and region of transplantation. However, even when these factors are kept constant, the amount and composition of infiltrating mouse cells are highly variable, which makes accurate molecular downstream analyses difficult. The contaminating mouse cells lead to cross-hybridization of mouse-derived molecules to human probes on microarrays and a significant reduction of sensitivity caused by measurement of mouse signals during next-generation sequencing or proteome analysis. In addition, the culture of human tumor cells is frequently hampered by murine fibroblasts overgrowing the target cells. For optimal results, the Mouse Cell Depletion Kit should be used in combination with the Tumor Dissociation Kit, human (# 130-095-929).
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LS or autoMACS® Columns.
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