Target Name
Fibroblast Growth Factor 23 (FGF23)
Abbreviation
FGF23
Species
Bos taurus (Bovine)
Sample Types
serum, plasma
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays):CV%<10% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
95 |
|
|
| Range % |
86-99 |
|
|
| 1:2 |
Average % |
103 |
|
|
| Range % |
97-107 |
|
|
| 1:4 |
Average % |
91 |
|
|
| Range % |
81-94 |
|
|
| 1:8 |
Average % |
100 |
|
|
| Range % |
90-105 |
|
|
Recovery
| The recovery of bovine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
104 |
94-108 |
|
|
| EDTA plasma (n=4) |
89 |
84-94 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 1000 |
1.928 |
1.952 |
1.940 |
1.809 |
|
|
| 500 |
1.402 |
1.324 |
1.363 |
1.232 |
|
|
| 250 |
0.878 |
0.862 |
0.870 |
0.739 |
|
|
| 125 |
0.553 |
0.540 |
0.547 |
0.416 |
|
|
| 62.5 |
0.329 |
0.341 |
0.335 |
0.204 |
|
|
| 31.2 |
0.241 |
0.256 |
0.249 |
0.118 |
|
|
| 15.6 |
0.178 |
0.174 |
0.176 |
0.045 |
|
|
| 0 |
0.129 |
0.133 |
0.131 |
|
|
|
|
Storage
Store at 2-8°C. Please refer to protocol.
Menu
X
