Extended! Enjoy FREE SHIPPING on all orders of FreeStyle Libre from 1st to 30th June 2021! Simply enter the code FREESHIP in the shopping cart, or at checkout to claim this offer.

Order pick-ups are currently not available.

Face masks (click here) and hand sanitisers (click here) available.


Search Website


If you know the product code enter it here  

µMACS c-myc Isolation Kit

The µMACS and MultiMACS c-myc Isolation Kits facilitate the isolation of c-myc fusion proteins from different sources. The super-paramagnetic µMACS MicroBeads included in the kits are conjugated to an anti-c-myc monoclonal antibody, enabling fast and effective magnetic labeling of c-myc-tagged fusion proteins. The protocol allows for protein isolation within just 1.5 hours. No centrifugation or buffer removal steps are required. For research use only. Size: for 40 isolations

Brand: Miltenyi Biotec

Learn more

Product CodeProduct Product Description Price (Excl gst) Qty Add
MB130-091-123 µMACS c-myc Isolation Kit µMACS c-myc Isolation Kit Login for price  

Go to checkout

Detailed procedure
After cell lysis c-myc-tagged proteins are magnetically labeled with µMACS Anti-c-myc MicroBeads. The sample is loaded onto a MACS Column placed in the magnetic field of a µMACS or thermoMACS Separator in which the magnetically labeled c-myc-tagged and associated proteins are retained during the washing steps. After elution with denaturing elution buffer, the precipitated proteins can be analyzed by SDS-PAGE and Western blot. Alternatively, native proteins can be eluted by pH-shift using triethylamine pH 11.8 or by removing the column from the magnetic field and applying a suitable native elution buffer.

The µMACS and MultiMACS c-myc Isolation Kits facilitate direct immunoprecipitation of c-myc fusion proteins from cell lysates at an analytical scale. They can also be used in co-immunoprecipitation experiments for the isolation of associated proteins or protein complexes in protein-protein interaction studies.

Downstream applications
The (co-)immunoprecipitated proteins can be analyzed by SDS-polyacrylamide gel electrophoresis, Western blot analysis, or mass spectrometry. Enzymatic on-column assays can also be performed using the thermoMACS Separator.

Browsing? Download Our Current Promotions and See Where You Can Save