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Cellometer Auto 2000

The Cellometer Auto 2000 is an automated instrument that uses flow cytometry and image analysis to count cells in a sample. The Cellometer Auto 2000 is capable of handling multiple samples at once and can count a wide range of cell sizes and types, including cancer cells, stem cells, and primary cells.

Brand: Nexcelom

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Primary Cell Analysis: PBMCs, Stem Cells, and more

The Cellometer Auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including:

  • Nucleated Cells for Transplantation
  • PBMCs for Immunology
  • Splenocytes for Vaccine Development
  • Stem Cells for Cellular Therapy
  • Tumor Cell Suspensions for Oncology

Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both ‘messy’ and ‘clean’ samples enables the Auto 2000 to evaluate samples at a variety of points throughout sample processing – from initial collection to separation, to cryopreservation.

The Cellometer Auto 2000 features one-touch assays for analysis of a wide range of primary samples, including:

Nucleated Cells from Whole Blood

Nucleated Cells from Whole Blood using Cellometer Auto 2000

Stem Cells

Stem Cells, image from Cellometer Auto 2000

PBMCs / Splenocytes following Separation

PBMCs / Splenocytes following Separation using Cellometer Auto 2000

Tumor and Tissue Cell Suspension

Tumor and Tissue Cell Suspension using Cellometer Auto 2000

Cellometer Auto 2000
Includes • Cellometer Auto 2000 Instrument with integrated computer and
touch-screen and dual-fluorescent optics
• Cellometer Software
• Two USB 2.0 ports
• Power Supply
• Ethernet port
• Phone/online applications support during set-up
Imaging Performance Cell Size: 5 – 300* microns
Conc. Range: 105 – 107 cells/mlBright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
*Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter
Instrument Specifications Weight: 26 lbs. (11.8 kg)
Dimensions: Width: 11.1″ (28.3 cm)
Depth: 12.8″ (32.4 cm)
Height: 13.4″ (34.0 cm)Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.5A
Output to Instrument: 12 VDC, 7.5A
Display 10.4″ touch screen
Fluorescent Optics Excitation / Emission: 470 nm/535 nm
Example Fluorophores:
• Acridine Orange (+DNA)
• Calcein AM
• CFDA
• SYTO® 9
• SYTO® 13Excitation / Emission: 540 nm/605 nm
Example Fluorophores:
• Propidium Iodide
• Ethidium Bromide