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Celigo Image Cytometer

Celigo is a plate-based benchtop brightfield and fluorescent imaging system designed for whole-well live-cell analysis and cell sample characterization. Celigo images and analyzes cells in various types of vessels including 6 – 1536 well plates, T25, T75 flasks, 10 cm dishes, and glass slides without disturbing their natural state. Individual cell level analysis is easily generated, providing cell level insights unlike ELISA or protein-based assays, and at a faster rate than flow cytometry. A broad range of complex cell-based assays have been optimized for Celigo: Apoptosis | Cell Cycle | Fluorescent Reporters | Cytotoxicity | Label-free Proliferation

Brand: Nexcelom

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Rapid Whole-well Imaging and Analysis

  • Sensitive whole-well imaging ensures accurate cell population analysis
    • Remove non-uniform seeding from the equation
    • Accurately detect, image, and count every cell in every well
  • Unique scanning mirrors enable fast, high-quality images

    Best-in-Class Brightfield and Four Fluorescent Channels

    • Proprietary algorithm for simultaneous imaging and label-free cell analysis
    • Perform real-time multiplex assays with four fluorescent channels

    Convenient Workflow Designed for Biologists

    • Multiple object-driven algorithms and a variety of assay-based applications
      • Accessible adaptation with easy-to-follow software
      • Save customizable experiments as well as default settings
      • Built-in gating parameters allow easy data analysis and visualization
      • Real-time graphic feedback allows multiple parameters to be measured simultaneously for intuitive analysis:
        • Cell growth | Viability | Morphology | Count | Concentration

    Automation and Data Management

    • Accessible application programming interface (API) for easy automated workflow integration
    • Automated microplate handling for kinetic end-point analysis or time-point analysis


  • Proprietary image acquisition and processing software
  • Powerful analysis software/Dell Precision computer
  • Windows 10


  • 1 LED-based enhanced brightfield imaging channel with uniform well illumination
  • 4 LED-based fluorescent channels
  • Proprietary F-theta lens with superior well edge-to-edge contrast
  • Galvanometric mirrors for fast imaging of large areas
  • Large chip CCD camera (2024 x 2024 pixels)
  • 1, 2, 4 or 8 μm/pixel resolution

Fluorescent Channels

Channel Excitation Dichroic Emission Typical Dyes
Blue 377/50 409 470/22 Hoechst, DAPI
Green 483/32 506 536/40 FITC, Calcein, GFP,
AlexaFluor® 488
Red 531/40 593 629/53 R-PE, PI, Texas Red, AlexaFluor® 568
Far-Red 628/40 660 688/31 DRAQ5®, AlexaFluor® 647

Plate Compatibility

  • 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
  • T-25 and T-75 flasks
  • Slides and cell arrays plate profiles available upon request

High-Speed Imaging

  • Less than 2 minutes per 384-well plate

Weight and Dimensions

  • Dimensions: 19.5″W x 16″H x 24″D (49.5cm x 40cm x 61cm)
  • Weight: 117 lbs. (53 kg)

Power Requirements

  • 110-220 VAC 50-60 Hz

Regulatory Compliance

  • CE marking