µ Columns can be used with the following separators:
Column capacity µ Columns are used for cell lysates from up to 107 cells.
Background information µ Columns are especially designed for small-scale molecular biology and protein biochemistry applications. Prior to use, the µ Column is placed in a separator, which induces a strong magnetic field within the column. Molecules labeled with the extremely small MACS® MicroBeads are retained on the column, whereas unlabeled material can be efficiently washed away. Additionally, enzymatic processing of retained molecules, such as cDNA synthesis, can be performed on the µ Column when placed in the thermoMACS™ Separator. Target molecules can be eluted in a small, convenient volume. Applications using µ Columns include mRNA isolation and cDNA synthesis, immunoprecipitation, purification of DNA and RNA binding proteins, protein interaction studies, or display technologies. µ Columns are individually packed in a sterile way.
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