NEXTFLEX^® Rapid DNA-Seq Library Prep Kit for Illumina^® Platforms

• Flexible amounts of input DNA from 1 ng to 1 µg
• Fast workflow
• Flexible barcode options – up to 384 single-index barcodes available
• Compatible with Illumina^® sequencing platforms including the MiSeq^®, HiSeq^®, NextSeq^® and HiSeq^® X Ten

We recommend the new NEXTFLEX^® Rapid DNA-Seq Kit 2.0 for your DNA-seq library prep needs.

Rapid DNA Library Prep for Sequencing on Illumina^® Platforms

The NEXTFLEX^® Rapid DNA-Seq Kit is a rapid DNA library prep kit producing libraries for Illumina^® sequencing from as little as 1 ng of DNA in two hours. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, upstream target capture samples, pooled amplicons and low input clinical samples. This DNA-Seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of input DNA amounts from 1 ng to 1 µg. The optimized protocol allows for fast generation of non-size-selected libraries and size-selected libraries using reproducible gel-free double SPRI selection.

Flexible Multiplexing Options

The NEXTFLEX^® Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. We strives to make available flexible barcoding options. The NEXTFLEX^® Rapid DNA-Seq Kit is designed to be used with the NEXTFLEX^® Unique Dual Index barcodes, NEXTFLEX^® DNA barcodes, NEXTFLEX-96^™ DNA barcodes and NEXTFLEX-HT^™ barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.

When less than 10 ng of starting material is available, the NEXTFLEX^® ChIP-Seq barcodes and NEXTFLEX-96^™ ChIP-Seq barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.

Cost Effective NEXTFLEX^® Rapid DNA-Seq Kit Bundles

To simplify your DNA sequencing PerkinElmer offers affordable bundles containing NEXTFLEX^® Rapid DNA-Seq library prep reagents and NEXTFLEX^® DNA barcodes. There are two different versions of this kit. They each contain the 48 rxn NEXTFLEX^® Rapid DNA-Seq Kit and 24 unique NEXTFLEX^® DNA Barcodes (in aliquots of 2 reactions each).

Automation Compatibility

The NEXTFLEX^® Rapid DNA-Seq Library Prep Kit was designed for easy migration onto automated liquid handling platforms. A validated automation protocol for use with the PerkinElmer Sciclone^® G3 NGS and NGSx workstations is available.

KIT CONTENTS

• NEXTFLEX^® End-Repair & Adenylation Buffer Mix
• NEXTFLEX^® End-Repair & Adenylation Enzyme Mix
• NEXTFLEX^® Ligase Enzyme Mix
• NEXTFLEX^® PCR Master Mix
• Nuclease-free Water
• NEXTFLEX^® Sizing Solution
• NEXTFLEX^® Resuspension Buffer

REQUIRED MATERIALS NOT PROVIDED

• 1 ng – 1 µg of fragmented DNA in up to 32 µL nuclease-free water.
• NEXTFLEX^® DNA Barcodes – 6 / 12 / 24 / 48 (Cat # 514101, 514102, 514103, 514104) or NEXTFLEX-96^™ DNA Barcodes (Cat # 514106)
• Ethanol 100% (room temperature)
• Ethanol 80% (room temperature)
• 96 well PCR Plate Non-skirted (Phenix Research^®, Cat # MPS-499) or similar
• 96 well Library Storage and Pooling Plate (Fisher Scientific^®, Cat # AB-0765) or similar
• Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
• Agencourt^® AMPure^® XP 60 mL (Beckman Coulter Genomics^®, Cat # A63880)
• Magnetic Stand -96 (Thermo Fisher^® Scientific, Cat # AM10027) / or / similar
• Thermocycler
• 2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
• Nuclease-free barrier pipette tips
• Vortex