Next Gen Sequencing Kits 

Features

• Explore our NEW! NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 for an updated chemistry that delivers high performance.
• 2 hours faster than traditional Illumina® stranded RNA-Seq library prep protocols
• Provides precise measurement of strand orientation (>99%)
• >10 ng – 1 µg total RNA for enrichment by NEXTFLEX® Poly(A) Beads 2.0 or ~ 1 ng – 100 ng isolated mRNA or rRNA-depleted RNA
• Minimal hands-on time required
• Streamlined protocol reduces sample loss
• Utilizes dUTP-based methodology
• Robust, reliable performance
• Up to 96 barcodes available for multiplexing
• Automation protocols are available for the Sciclone® NGS and NGSx workstations
• Functionally validated with Illumina® sequencing platforms
• Allows for single read or paired end transcriptome sequencing

KIT CONTENTS

• NEXTFLEX® RNA Fragmentation Buffer
• NEXTFLEX® First Strand Synthesis Primer
• NEXTFLEX® Directional First Strand Synthesis Buffer Mix
• NEXTFLEX® Rapid Reverse Transcriptase
• NEXTFLEX® Directional Second Strand Synthesis Mix
• NEXTFLEX® Adenylation Mix
• NEXTFLEX® Ligation Mix
• NEXTFLEX® RNA-Seq Barcode Adapter 1 (0.6 µM)
• NEXTFLEX® Uracil DNA Glycosylase
• NEXTFLEX® Primer Mix (12.5 µM)
• NEXTFLEX® PCR Master Mix
• Nuclease-free Water
• Resuspension Buffer

REQUIRED MATERIALS NOT PROVIDED

• 10 ng – 1 µg total RNA for enrichment by NEXTFLEX® Poly(A) Beads 2.0 or ~ 1 ng – 100 ng isolated mRNA or rRNA-depleted RNA
• DynaMag™-2 Magnet
• Commercial kits for rRNA depletion (optional, if not using poly(A) enrichment)
• 100% Ethanol (stored at room temperature)
• 80% Ethanol ( freshly prepared )
• 2, 10, 20, 200 and 1000 µL pipettes
• RNase-free pipette tips
• Nuclease-free 1.5 mL microcentrifuge tubes
• Thin wall nuclease-free 0.5 mL microcentrifuge tubes
• 96 well PCR Plate Non-skirted 
• Adhesive PCR Plate Seal 
• Agencourt AMPure XP 60 mL
• Magnetic Stand -96
• Microcentrifuge
• Thermocycler
• Heat block
• Vortex