Next Gen Sequencing Kits 

Features

• Reliable: High coverage uniformity with low duplication rate
• Convenient: Optimized for use with 5 ng – 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included
• Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut™ rRNA depletion kit (human, mouse, rat)
• Flexible: Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run)
• Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations

New & Improved Library Preparation Kit for your RNA Sequencing Needs

The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0 (10 ng – 5 μg) or NEXTFLEX® RiboNaut™ rRNA depletion kit (human, mouse, rat) (5 ng – 1 μg). This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut™ rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes (6.25μM), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample.

KIT CONTENTS

• NEXTFLEX® RNA-Seq Fragmentation Buffer 2.0
• NEXTFLEX® Directional First Strand Synthesis Buffer Mix 2.0
• NEXTFLEX® Rapid Reverse Transcriptase 2.0
• NEXTFLEX® Directional Second Strand Synthesis Mix 2.0
• NEXTFLEX® RNA-Seq Adenylation Buffer Mix 2.0
• NEXTFLEX® RNA-Seq Adenylation Enzyme 2.0
• NEXTFLEX® RNA-Seq Ligase Buffer Mix 2.0
• NEXTFLEX® RNA-Seq Ligase Enzyme 2.0
• NEXTFLEX® RNA-Seq PCR Master Mix 2.0
• NEXTFLEX® RNA-Seq Primer Mix 2.0 (50 µM)
• Nuclease-free Water
• Resuspension Buffer
• NEXTFLEX® Cleanup Beads XP

REQUIRED MATERIALS NOT PROVIDED

• 10 ng – 1 µg total RNA for enrichment by NEXTFLEX® Poly(A) Beads or ~ 1 ng – 100 ng isolated mRNA or rRNA-depleted RNA
• DynaMag™-2 Magnet 
• 100% Ethanol (stored at room temperature)
• 2, 10, 20, 200 and 1000 µL pipettes
• RNase-free pipette tips
• Nuclease-free 1.5 mL microcentrifuge tubes
• Thin wall nuclease-free 0.5 mL microcentrifuge tubes
• 96 well PCR Plate Non-skirted
• Adhesive PCR Plate Seal
• Magnetic Stand -96
• Microcentrifuge
• Thermal cycler
• Heat block
• Vortex