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NEXTFLEX® Bisulfite-Seq Barcodes

The NEXTFLEX® Bisulfite-Seq Barcodes are designed to be used with the NEXTFLEX® Bisulfite-Seq Kit. Unlike other NEXTFLEX® Barcodes, the NEXTFLEX® Bisulfite-Seq Barcodes are methylated. The NEXTFLEX® Bisulfite-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX® Bisulfite-Seq Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. These methylated adapters can be used with single, paired-end and multiplex reads.

Brand: Revvity

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Product CodeProduct Product Description Price (Excl gst) Qty Add
PENOVA-511911 NEXTFLEX BISULFITE-SEQ BARCODES-6 48 RXN NEXTFLEX BISULFITE-SEQ BARCODES-6 48 RXN Login for price  
PENOVA-511912 NEXTFLEX BISULFITE-SEQ BARCODES-12 96RXN NEXTFLEX BISULFITE-SEQ BARCODES-12 96RXN Login for price  
PENOVA-511913 NEXTFLEX BISULFITE-SEQBARCODES-24 192RXN NEXTFLEX BISULFITE-SEQBARCODES-24 192RXN Login for price  

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NEXTFLEX® Bisulfite-Seq Barcodes

 

  • Up to 24 methylated adapters for multiplexing Illumina® RRBS and WGBS libraries are available
  • Considerably reduce your per-sample sequencing cost by barcoded multiplexing
  • Increase your sequencing scale by pooling samples on a single flow cell
  • Compatible with bisulfite-sequencing on the Illumina® sequencing platforms

 

Pool Multiple Library Preparations in a Single Flow Cell Lane

The NEXTFLEX® Bisulfite-Seq Barcodes are designed to be used with the NEXTFLEX® Bisulfite-Seq Kit. Unlike other NEXTFLEX® Barcodes, the NEXTFLEX® Bisulfite-Seq Barcodes are methylated. The NEXTFLEX® Bisulfite-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX® Bisulfite-Seq Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR.

These methylated adapters can be used with single, paired-end and multiplex reads.

 

KIT CONTENTS

 

  • NEXTFLEX® Bisulfite-Seq Adapters (25 µM)
  • NEXTFLEX® Primer Mix (12.5 µM)