Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using Western blot analysis.
Available Formats
- The Ready-To-Glow Secreted Luciferase Reporter System includes a promoterless plasmid and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.
- The Lenti-X Ready-To-Glow Secreted Luciferase Reporter System includes a promoterless vector with lentiviral delivery, and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.
- The NFkB Secreted Luciferase Reporter System is designed to monitor NFkappaB signal transduction pathways. Adding TNF-alpha, IL-1, or lymphokine receptor stimulants to the cell culture medium induces the binding of transcription factors to the enhancer element, thereby initiating transcription of the secreted Metridia luciferase reporter gene.
- The CRE Secreted Luciferase Reporter System is designed to monitor the activation of cAMP binding protein (CREB) and cAMP-mediated signal transduction pathways. For example, treatment of cells with cAMP or forskolin activates CREB or ATF to bind CRE, thus initiating transcription of the secreted Metridia luciferase reporter gene.
Dual Secreted Reporter Assay—Luciferase & SEAP
The Ready-To-Glow Dual Secreted Reporter System has two live cell reporters that do not require cell lysis. In addition to the secreted luciferase assay, it includes a secreted version of alkaline phosphatase.
Features
- Secreted luciferase assay protocol eliminates cell lysis
- 2–4 fold higher signal than firefly or Renilla luciferase
- Single transfection allows multiple time points from same cell
- Dual, live cell assay includes SEAP as well as secreted luciferase: monitor two promoters, or use one reporter as a normalization control
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