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  6. Great EscAPe™ SEAP

Great EscAPe™ SEAP

The Great EscAPe SEAP 2.0 System uses a secreted alkaline phosphatase assay to analyze cis-acting DNA sequences and trans-acting factors. The kinetics of gene expression can easily be studied with this secreted alkaline phosphatase assay by repeatedly sampling the same culture.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT631738 Great EscAPe™ SEAP Chemiluminescence Kit 2.0, 1,000 Rxns Great EscAPe™ SEAP Chemiluminescence Kit 2.0, 1,000 Rxns Login for price  
CLT631737 Great EscAPe™ SEAP Chemiluminescence Kit 2.0, 300 Rxns Great EscAPe™ SEAP Chemiluminescence Kit 2.0, 300 Rxns Login for price  
CLT631736 Great EscAPe™ SEAP Chemiluminescence Kit 2.0, 50 Rxns Great EscAPe™ SEAP Chemiluminescence Kit 2.0, 50 Rxns Login for price  

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The kit has an extremely low background signal, with a wide dynamic range and high sensitivity. Furthermore, the robust signal is extremely stable over time. Cells can be studied further after the secreted alkaline phosphatase assay using Northern blots, RNase protection assays, Western blots and other methods.

Features

  • One step assay, for monitoring promoter activity
  • 10-fold higher sensitivity then firefly luciferase
  • No-cell-lysis protocol
  • Adaptable from single-tube to high-throughput assays
  • Dual, live cell assay includes secreted luciferase as well as SEAP: monitor two promoters, or use one reporter as a normalization control