NEXTFLEX^® ChIP-Seq Library Prep Kit for Illumina^® Sequencing

• Optimized for down to 1 ng of DNA input
• For use with ChIP or genomic DNA samples
• Optimized for low DNA input
• Enhanced Adapter Ligation Technology offers optimal coverage and unique reads
• Flexible barcode options – up to 96 unique, single-index barcoded adapters or 1,536 UDI barcodes available
• Compatible with the Illumina^® MiSeq^®, HiSeq^®, HiSeq^®, HiSeq^® X Ten, and NextSeq^® 500 sequencing platforms

esigned for Ultra-low DNA Input

The NEXTFLEX^® ChIP-Seq Kit is a library prep kit specifically designed for ultra-low DNA input Illumina^® sequencing applications. As little as 1 ng of chromatin immunoprecipitated or genomic DNA is required to produce viable sequencing libraries compatible with single-read or paired-end sequencing.

The NEXTFLEX^® ChIP-Seq Kit also features proprietary Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads and increased yields from minimal quantities of starting material. The NEXTFLEX^® ChIP-Seq Kit now incorporates a bead-based size selection protocol, which completely eliminates the gel purification step. Additionally, there is an optional protocol which eliminates the size selection step entirely.

Unparalleled Multiplexing Capabilities

Using the NEXTFLEX^® ChIP-Seq Barcodes, the user can index 6, 12, 24, 48, or even 96 samples at once providing unparalleled Illumina^® ChIP sequencing capacity.

KIT CONTENTS

• NEXTFLEX^® ChIP End Repair Buffer Mix
• NEXTFLEX^® ChIP End Repair Enzyme Mix
• NEXTFLEX^® ChIP Adenylation Mix
• NEXTFLEX^® ChIP Ligation Mix
• NEXTFLEX^® ChIP Adapter (0.6 µM)
• NEXTFLEX^® ChIP Primer Mix (12.5 µM)
• NEXTFLEX^® ChIP PCR Master Mix
• Nuclease-free Water
• Resuspension Buffer
• ChIP-Seq DNA Control

REQUIRED MATERIALS NOT PROVIDED

• 1 ng – 10 ng of DNA in up to 40 µL nuclease-free water
• NEXTFLEX^® ChIP-Seq Barcodes – 6 / 12 / 24 / 48 or NEXTFLEX-96^™ ChIP-Seq Barcodes (Cat # 514120, 514121, 514122, 514123, 514124)
• Ethanol 100% (room temperature)
• Ethanol 80% (room temperature)
• Covaris System (S2, E210) or other device for fragmenting DNA
• 96 well PCR Plate Non-skirted (Phenix Research^®, Cat # MPS-499) / or / similar
• 96 well Library Storage and Pooling Plate (Fisher Scientific^®, Cat # AB-0765) / or / similar
• Adhesive PCR Plate Seal (Bio-Rad^®, Cat # MSB1001)
• Agencourt^® AMPure^® XP 5 mL (Beckman Coulter Genomics^®, Cat # A63880)
• Magnetic Stand -96 (Thermo Fisher^® Scientific, Cat # AM10027) / or / similar
• Heat block
• Thermocycler
• 2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
• Nuclease-free barrier pipette tips
• Microcentrifuge
• 1.5 mL nuclease-free microcentrifuge tubes
• Vortex