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The NEXTFLEX® Bisulfite-Seq Kit is a versatile kit designed to facilitate assessment of the methylation state of the genome and simplify workflow by using master mixed reagents and magnetic bead based cleanup to reduce pipetting and eliminate time-consuming steps in library preparation. In addition, the availability of up to 24 methylated NEXTFLEX® Bisulfite-Seq barcoded adapters makes multiplexing simple. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTFLEX® ligation enzymatic mix allows users to perform ligations with longer adapters and superior ligation efficiencies.
Brand: Revvity
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The NEXTFLEX® Bisulfite-Seq Library Prep Kit is designed for both reduced representation bisulfite sequencing (RRBS) and whole-genome bisulfite sequencing (WGBS). It generates single, paired-end and multiplexed DNA libraries for sequencing on Illumina® platforms. The NEXTFLEX® U+ Polymerase Mix included in the kit is able to read through uracil present in DNA templates and has been optimized to amplify bisulfite converted templates.
RRBS: 10 ng of Msp1 digested DNA input, 18 PCR cycles, 10 nm yield
RRBS: 1 ug Msp1 digested DNA input, 18 PCR cycles, 32 nm yield
WGBS: 10 ng of 200 bp sheared DNA input, 18 PCR cycles, 58 nm yield
WGBS: 1 ug 200 bp sheared DNA input, 15 PCR cycles, 28 nm yield
The NEXTFLEX® Bisulfite-Seq Kit is a versatile kit designed to facilitate assessment of the methylation state of the genome and simplify workflow by using master mixed reagents and magnetic bead based cleanup to reduce pipetting and eliminate time-consuming steps in library preparation. In addition, the availability of up to 24 methylated NEXTFLEX® Bisulfite-Seq barcoded adapters makes multiplexing simple. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTFLEX® ligation enzymatic mix allows users to perform ligations with longer adapters and superior ligation efficiencies.
The NEXTFLEX® Bisulfite-Seq Kit with reduced representation utilizes a restriction enzyme that leads to selective amplification of CpG regions, resulting in focused sequence depth. The NEXTFLEX® Msp1 Restriction Enzyme, which is available separately, has been optimized for reduced representation studies using the NEXTFLEX® Bisulfite-Seq Kit. The NEXTFLEX® Bisulfite-Seq Kit can also be used without the MSP 1 Restriction Enzyme for genome-wide methyl-seq analysis, including under-represented CpG regions.
KIT CONTENTS
REQUIRED MATERIALS NOT PROVIDED
