5’-3’ Thermostable DNA Polymerase isolated from the extreme thermophilic bacteria Thermus thermophilus YT1. Taq DNA Polymerase is a thermostable enzyme which replicates DNA at 74C and remains functional after incubation at 95C. The ability of this enzyme to survivemultiple rounds of temperature cycling is the basis of the PCR reaction and thermal cycle sequencing. It is supplied with 25mM MgCl2 (1ml) and 10x PCR Reaction Buffer (1ml).This enzyme can undergo several freeze/thaw cycles without loss of activity. This allows priorpreparation of complete amplification mixtures (minus target DNA) that can be stored at -20C or -80C in single tubes under paraffin oil providing convenient and consistent reactions and assisting in minimising problems due to cross contamination.
Concentration: 5 units/µl
(One unit is the amount of enzyme that is required to incorporate 10 nmoles of dNTPs into acid in soluble material at 70C in 30 minutes using 'activated' herring sperm DNA as substrate.)
10x Reaction Buffer: 670mM Tris-HCl (pH 8.8 at 25C), 166Mm (NH4)2SO4, 4.5% Triton X-100, and 2mg/ml Gelatin.
Applications: PCR-based DNA amplification, cycle sequencing, amplification of cloned inserts
in vectors, and primer extensions.
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