Applications
- Purification of human or animal viral RNA and DNA from serum, plasma, swab, and tissue homogenates
Features
- Convenient and highly efficient sample lysis by liquid Proteinase K
- Reliable virus detection from fresh or frozen serum / plasma treated with EDTA / citrate
- Highest sensitivity for DNA and RNA viruses e.g., Blue Tongue Virus and Cytomegalovirus
Specifications
- Technology: Silica membrane technology
- Fragment size: 100 bp–approx. 50 kbp
NucleoSpin® 8 Virus
- Processing: Manual or automated
- Sample material: Biological fluids (< 150 μL)
- Typical recovery: > 90 %
- Elution volume: 70–100 μL
- Binding capacity: 40 μg
- Processing time: 60 min/48 preps
NucleoSpin® 96 Virus
- Processing: Manual or automated
- Sample material: Cell-free biological fluids (< 150 μL)
- Typical recovery: > 90 %
- Elution volume: 70–100 μL
- Binding capacity: 40 μg
- Processing time: 60 min/96 preps
Proportional detectability of viral DNA/RNA even at low titers
Nucleic acids were extracted from dilution series of DNA (blue) and RNA (orange) viruses and quantified by qPCR. Both viral DNA and viral RNA were detected with high sensitivity (down to 100 viral particles/µL for DNA; down to 800 viral particles/µL for viral RNA).
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