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Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification
Brand: Macherey-Nagel
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Features
Product
Preps
Specification
REF
Protino® Ni-NTA Columns 1 mL
5
FPLC™ columns
MN745410.5
Protino® Ni-NTA Columns 5 mL
1
MN745415.1
as above
MN745415.5
Technology
IMAC (immobilized metal ion affinity chromatography)
Chelating ligand
NTA (nitrilotriacetic acid)
Format
1 mL FPLC™ columns
5 mL FPLC™ columns
Column dimensions
0.7 cm (inner diameter) x 2.5 cm (height)
1.6 cm (inner diameter) x 2.5 cm (height)
Column body material
Polypropylen
Column inlet port
10-32 (1/16'') female
Column outlet port
10-32 (1/16'') male
Matrix
6 % beaded agarose (cross-linked), precharged with Ni2+
Bead size
45–165 µm
Binding capacity*
50 mg
250 mg
Recommended flow rate**
1 mL/min
5 mL/min
Max. recommended flow rate
4 mL/min
10 mL/min
Storage solution
30 vol % ethanol
Storage temperature
4–8 °C
* Binding capacity will vary for each polyhistidine-tagged protein ** High flow rates may reduce binding capacity